Fig. 3: RNF180 promotes the degradation of RhoC protein by increasing its ubiquitination level in gastric cancer cells. | Cell Death & Disease

Fig. 3: RNF180 promotes the degradation of RhoC protein by increasing its ubiquitination level in gastric cancer cells.

From: RNF180 mediates STAT3 activity by regulating the expression of RhoC via the proteasomal pathway in gastric cancer cells

Fig. 3

A, B GC cells were transfected by either RNF180 plasmid or control vector. After 36 h, cells were treated with 100 μg/ml CHX at the indicated time point. The cell lysates were subjected to immunoblotting and RhoC expression was quantified by ImageJ software. C, D Ubiquitination assay was performed with 5 μM MG132 for 11 h in order to further explore the ubiquitination degradation of RhoC and pSTAT3 by RNF180 in GC cells.

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