Fig. 3: Gene expression is differentially modulated by EVI1-WT and EVI1-S436A.
From: EVI1 phosphorylation at S436 regulates interactions with CtBP1 and DNMT3A and promotes self-renewal
a Experimental design. Murine haematopoietic c-Kit+ stem and progenitor cells (KIT+ HSPCs) cells were isolated and transduced with Evi1-WT and Evi-S436. 48 h after transduction RNA was extracted and processed for RNAseq analysis. b Two-way RNAseq analysis of Evi1-WT vs vector-only and Evi1-S436A vs vector-only transduced KIT+ HSPCs. Venn diagram of transcripts significantly changed by Evi1-WT or Evi1-S436A (adj. p > 0.05, FC > 0.6). c Heatmap illustration of selected differentially expressed genes in the two-way comparison (black triangle: adj.p < 0.05) d Regression analysis comparing fold change of significantly (p < 0.05) changing transcripts in Evi1-WT and Evi1-S436A transduced cells. e Heatmap illustration of group-ANOVA analysis of vector-only-, Evi1-WT - and Evi1-S436 transduced KIT+ HSPCs, adj. p value range of 2.3e−8 to <0.01. f, g Hypernetwork heatmap for Evi1-WT (n = 78 transcripts, mean connectivity = 2376) and Evi1-S436A (n = 106 transcripts, mean connectivity = 3561), respectively. Colour intensity represents number of binary relationships shared between a pair of transcripts with the rest of the transcriptome (n = 23766 total transcripts) h Shannon entropy in Evi1-WT (n = 78) and Evi1-S436A (n = 106) induced hypernetworks (p < 2.2e−16).
