Fig. 4: Mapping of nuclear localization signal sites in RFPL3. | Cell Death & Disease

Fig. 4: Mapping of nuclear localization signal sites in RFPL3.

From: Importin 13 promotes NSCLC progression by mediating RFPL3 nuclear translocation and hTERT expression upregulation

Fig. 4

A The predicted nuclear localization sequences of RFPL3 using bioinformatics software cNLS Mapper. B Schematic of RFPL3 protein and its truncation mutants tagged with FLAG. C A549 cells transfected with RFPL3-FLAG-conjugated plasmids. Forty-eight hours later, cells stained with Flag antibody; then immunofluorescent assay was performed. Scale bar, 50 μm. D Flag-tagged RFPL3 and mutants were transiently transfected. Forty-eight hours post transfection, cells were lysed and immunoprecipitated with anti-IPO13 or rabbit IgG control and blotted with anti-Flag.

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