Fig. 1: SKA3 is highly expressed in laryngeal squamous cell carcinoma (LSCC) with a poor prognosis. | Cell Death & Disease

Fig. 1: SKA3 is highly expressed in laryngeal squamous cell carcinoma (LSCC) with a poor prognosis.

From: Targeting SKA3 suppresses the proliferation and chemoresistance of laryngeal squamous cell carcinoma via impairing PLK1–AKT axis-mediated glycolysis

Fig. 1

A Transcriptome sequencing of 53 pairs of LSCC and matched adjacent normal mucosal (ANM) tissues was performed to identify the top 50 upregulated genes in LSCC for cluster analysis. B The top 50 upregulated genes were individually knocked down via infection with shRNA viruses in the LSCC cell line FD-LSC-1; cell proliferation was measured using a high-content screening system for 5 days. Top: fold change of the cell proliferation of each group normalized to day 1. Bottom: representative cell images from high-content screening. NC negative control, PC positive control. Scale bar, 200 μm. C SKA3 expression in paraffin sections of an LSCC tissue array (n = 165) was detected using IHC. Representative images of IHC and standard of IHC scoring are shown. Scale bar, 20 μm. D The SKA3 protein level in LSCC and ANM tissues was determined using IHC. E–I Association analysis of the SKA3 protein levels with T stage (E), clinical stage (F), degree of pathological differentiation (G), N stage (H), and M stage (I) of LSCC. J Kaplan–Meier analysis of the association of the SKA3 levels with the overall survival of patients with LSCC. The SKA3 expression levels were divided into low or high groups according to the median IHC score (n = 165). K RT–qPCR and western blot analysis of endogenous SKA3 in MRC-5, TU-177, and FD-LSC-1 cells. The data are expressed as means ± SD of three independent experiments in (B) and (K).

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