Fig. 2: SKA3 promotes laryngeal squamous cell carcinoma (LSCC) proliferation and growth of LSCC xenograft tumors. | Cell Death & Disease

Fig. 2: SKA3 promotes laryngeal squamous cell carcinoma (LSCC) proliferation and growth of LSCC xenograft tumors.

From: Targeting SKA3 suppresses the proliferation and chemoresistance of laryngeal squamous cell carcinoma via impairing PLK1–AKT axis-mediated glycolysis

Fig. 2

A Western blot analysis of SKA3 expression in SKA3-knockdown (left) or SKA3-overexpressing (right) FD-LSC-1 and TU-177 cells. B Overexpression of SKA3 promoted colony formation by the FD-LSC-1 and TU-177 cell lines (upper), while silencing of SKA3 expression suppressed colony formation (lower). C, D FD-LSC-1 and TU-177 cells were transfected with siRNAs targeting SKA3 (SKA3-KD), negative-control siRNAs (NC) (C), SKA3-overexpression plasmid (SKA3-OE), or empty vector (vector) (D). Twenty-four hours after transfection, the cells were reseeded in real-time cell analysis (RTCA) plates or 96-well plates, and proliferation was measured using the RTCA system and EdU staining (merged images are superimposed on the RTCA curve). E, F Silencing (E) or overexpression (F) of SKA3 inhibited or promoted the growth, respectively, of tumors generated by xenografted FD-LSC-1 and TU-177 LSCC cell lines in nude mice. Tumor growth curves and end-point weights are shown (upper). Representative images (lower) are displayed (six mice per group). G, H IHC analysis of the expression of SKA3 and the marker of proliferation Ki67 in sections of tumors generated by xenografted FD-LSC-1 (G) and TU-177 (H) cells. Representative IHC images (left) and semiquantitative analysis of IHC images using QuPath software (right) are displayed. Scale bar, 50 µm. The data are expressed as means ± SD of three independent experiments.

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