Fig. 3: SKA3 promotes glycolysis in laryngeal squamous cell carcinoma (LSCC) cells. | Cell Death & Disease

Fig. 3: SKA3 promotes glycolysis in laryngeal squamous cell carcinoma (LSCC) cells.

From: Targeting SKA3 suppresses the proliferation and chemoresistance of laryngeal squamous cell carcinoma via impairing PLK1–AKT axis-mediated glycolysis

Fig. 3

A RNA-seq was performed on SKA3-knockdown (SKA3-KD) TU-177 cells. GO analysis of downregulated genes in SKA3-KD TU-177 cells; the glycolytic process is indicated with an asterisk. B KEGG pathway analysis of downregulated genes in SKA3-KD TU-177 cells. C, D Glycolysis capacity in SKA3-knockdown or SKA3-overexpressing FD-LSC-1 (C) and TU-177 cells (D) was measured using a Seahorse energy metabolism instrument (Seahorse XFP). The ECAR value indicates the rate of lactic acid production during glycolysis. E Knockdown or overexpression of SKA3 in FD-LSC-1 and TU-177 LSCC cells. The expression levels of HK2, PFKFB3, and PDK1 were determined using western blot analysis. F Representative whole-mount IHC staining images of SKA3, HK2, PFKFB3, and PDK1 in the embryos of Ska3-WT (+/+) and KO (+/−) mice on day 16.5 of gestation. Scale bar, 2 mm. The data are expressed as means ± SD of three independent experiments in (C) and (D). *P < 0.05; **P < 0.001.

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