Fig. 6: miR-322/-503 directly bound and gradually degraded the expanded CUG repeats. | Cell Death & Disease

Fig. 6: miR-322/-503 directly bound and gradually degraded the expanded CUG repeats.

From: miR-322/-503 rescues myoblast defects in myotonic dystrophy type 1 cell model by targeting CUG repeats

Fig. 6

miR-322 (A) and miR-503 (B) displayed colocalization with the expanded CUG repeats via RNA FISH. The cells at 8 and 48 after Dox induction were used for the RNA FISH staining. C GFP mRNA level was measured at 0, 6, 12, 24, 48 h after transient transfection of GFP-CUG200 and miR-322/-503 overexpression plasmids in HEK293T cells through RT-qPCR. All expression levels were normalized to the 0-h group. D The levels of SEMA3A, CDC25A, and IGF1R were determined by RT-qPCR in both C2C12-CUG200/pLL4.0-miR-322/-503 and C2C12-CUG5/pLL4.0-miR-322/-503 cells. All expression levels were normalized to the C2C12-CUG5/pLL4.0-miR-322/-503 group. * statistically significant (p < 0.05); ns not statistically significant.

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