Fig. 3: Analysis of ROS and lipid ROS during non-canonical pyroptosis in genetic models targeting the pyroptosis pathway. | Cell Death & Disease

Fig. 3: Analysis of ROS and lipid ROS during non-canonical pyroptosis in genetic models targeting the pyroptosis pathway.

From: Excessive phospholipid peroxidation distinguishes ferroptosis from other cell death modes including pyroptosis

Fig. 3

a–b Cell death and lipid ROS accumulation in casp11−/− BMDMs and WT 8 h after LPS transfection of LPS-primed cells. c–e Cell death, lipid ROS and ROS in gsdmd−/− BMDMs during non-canonical pyroptosis. f Cleavage of WT and gsdmdI105N/I105N. g–h Lipid ROS and cell death of gsdmdI105N/I105N during non-canonical pyroptosis. i Representative fluorescent microscopy images of WT, casp11−/−, gsdmd−/− and gsdmdI105N/I105N stained with C11-BODIPY and DRAQ7 5 h after LPS transfection. All bars are mean ± SEM of three biological replicates. One-way ANOVA, *p < 0.05, **p < 0.01.

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