Fig. 4: The effect of Fer1 on ferroptosis and non-canonical pyroptosis execution. | Cell Death & Disease

Fig. 4: The effect of Fer1 on ferroptosis and non-canonical pyroptosis execution.

From: Excessive phospholipid peroxidation distinguishes ferroptosis from other cell death modes including pyroptosis

Fig. 4

a, b Lipid ROS accumulation during ferroptosis (0.5 μM ML162) and non-canonical pyroptosis (3 h of 1 µg/ml LPS followed by 8 h incubation after LPS transfection) in the presence of 0.5 µM Fer1. c, d Cell death levels during ferroptosis and non-canonical pyroptosis in the presence of Fer1. e–f Representative fluorescent microscopy images from BMDMs undergoing different modes RCD showing the impact of Fer1 on lipid ROS and cell death. g The impact of Fer1 on ROS production during ferroptosis and non-canonical pyroptosis. h The impact of Fer1 on production of pro-inflammatory cytokines. Heatmaps represent values from three biological replicates. All bars are mean ± SEM of three biological replicates. One-way ANOVA, *p < 0.05, **p < 0.01, p < 0.0001.

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