Fig. 4: Flow cytometry verified that administration sPD-L1 reduced lung monocyte-derived macrophages.

A Gate strategy for dendritic cells, monocyte-derived macrophages, neutrophils and alveolar macrophages (MDMs were measured in lung tissue single-cell suspension while alveolar macrophages were assayed in BALF) (n = 9–12/each group). B Percentage of different cell populations in CD45-positive cells of lung or BALF (n = 9–12/each group). C Percentage of different cell populations in CD45-positive cells of blood. D PD-1 expression on MDMs of lung (n = 9–12/each group). E Representative histograms of PD-1 expression on MDM of lung. The cells were previously gated by CD45⁺CD11b⁺F4/80⁺ (n = 3–6/each group). The experiments have been repeated for at least three times. Each value represents the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, analyzed by t test. MDM monocyte-derived macrophages, Neu, neutrophil.