Fig. 6: Administration of sPD-L1 induced apoptosis of monocyte-derived macrophages.
From: Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages
A Representative histograms of PD-1 expression on monocyte-derived macrophages of healthy controls and ARDS patients. B PD-1 expression on MDM of healthy controls, Non-ARDS patients and ARDS patients. (n = 5/group) C The apoptosis of macrophages by PI/Annexin-V staining. D Apoptosis of macrophages in healthy controls, Non-ARDS patients and ARDS patients. (n = 5/group), analyzed by annexin V-fluorescein isothiocyanate/PI double staining. Cells in the B2 and B4 quadrants(annexin V + /PI+ and annexin V + /PI−, respectively) were considered to be apoptotic. D Graphical representation of apoptosis (n = 5/group). Each value represents the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, analyzed by t test. MDM monocyte-derived macrophages; HC healthy controls.
