Fig. 1: GDC-0349 potently inhibits NSCLC cell viability, proliferation, cell cycle progression, migration and invasion.

A549 cells (a–f) or the primary human NSCLC cells (NSCLC-1/-2/-3) (g–i) were treated with applied concentrations of GDC-0349 (5–500 nM) or the vehicle control (“Ctrl”, same for all Figures), cells were further cultured for applied time periods, cell viability (CCK-8 assay, a and g), colony formation (b), proliferation (nuclear EdU incorporation, c and h), cell cycle progression (PI-FACS, d), migration (“Transwell” assay, e and i) and invasion (“Matrigel Transwell” assay, f) were tested by assays mentioned in the text, and results were quantified. For nuclear EdU staining assays, five random views (1 × 100 magnification) with total 500 cells (for each condition) were included to calculate average EdU ratios (same for all figures). For “Transwell” and “Matrigel Transwell” assays, five random views for each condition were included to calculate the average number of migrated/invaded cells (same for all figures). The data are presented as mean ± standard deviation (SD, n = 5). *p < 0.05 vs. “Ctrl” cells. The experiments were repeated five times with similar results obtained. Bar = 100 μm (c, e, and f).