Fig. 3: TCTP mRNA knockdown cannot effectively reduce TCTP protein levels in HCC cells. | Cell Death & Disease

Fig. 3: TCTP mRNA knockdown cannot effectively reduce TCTP protein levels in HCC cells.

From: The mRNA of TCTP functions as a sponge to maintain homeostasis of TCTP protein levels in hepatocellular carcinoma

Fig. 3

A, B TCTP protein and mRNA expression levels in Huh7 cells after Different concentrations of siRNAs against TCTP were transfected with 48 h. GAPDH was used as an internal control. C PTB protein levels in Huh7 cells after different concentrations of siRNAs against TCTP were transfected with 48 h. D, E Different concentrations of siRNAs against TCTP were transfected into HL-7702 cells. 48 h later, western blot (D) and qPCR (E) were used to detect the TCTP protein and mRNA levels, respectively. F PTB protein levels after treatment with different doses of siRNA against PTB in HL-7702 cells. G TCTP protein levels after treatment with different doses of siRNA against TCTP in A549 cells. The experiments were performed in triplicate and GAPDH was used as an internal control for all experiments. The data are expressed as means ± sd. Statistical analyses using Student’s t test for independent groups were performed using GraphPad Prism5. *P < 0.05, **P < 0.01, ***P < 0.001.

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