Fig. 5: Low TCTP mRNA can enhance its translational efficiency. | Cell Death & Disease

Fig. 5: Low TCTP mRNA can enhance its translational efficiency.

From: The mRNA of TCTP functions as a sponge to maintain homeostasis of TCTP protein levels in hepatocellular carcinoma

Fig. 5

A, B The A HL-7702 or B Huh7 cells were treated with ActD (10 μg/ml) for different times, and western blot was used to detect the TCTP protein level in both cells. C, D The C HL-7702 or D Huh7 cells were treated with ActD (10 μg/ml) and CHX (10 μg/ml) for different times and western blot was used to detect the TCTP protein level in both cells. E, F The E HL-7702, and F Huh7 were treated with MG132 (1 μM) for a different time. Western blot was taken to detect the TCTP protein level in both cells. G The HL-7702 cells were pretreated with rapamycin (10 μM) for 1 h and then co-incubated with ActD (10 μg/ml) for a different time. Western blot was then taken to detect the TCTP protein level in cells. The experiments were performed in triplicate and actin was used as an internal control for all experiments.

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