Fig. 7: The formation of mitochondrial “spherical crista”.
From: OPA1 and MICOS Regulate mitochondrial crista dynamics and formation
A–C Live HeLa Mic10 KO (A), COS7 Mic10 KO (B), and OPA1 KO MEFs (C) cells were stained with MitoTracker Green (250 nM, 15 min), and then imaged by Hessian-SIM. The red arrowhead indicates the spherical crista. D Representative live-cell time-lapse images of mitochondria in COS7 Mic10 KO cells stained with MitoTracker Green (250 nM, 15 min). Time-lapse Hessian-SIM images reveal that two small mitochondria (the green and red arrowheads indicated) underwent the process of entering into a large mitochondrion. E Representative live-cell time-lapse Hessian-SIM images of mitochondria in COS7 Mic10 KO cells stained with MitoTracker Green (250 nM, 15 min). Time-lapse Hessian-SIM images reveal that a small mitochondrion (the red arrowhead indicated) entered into a giant mitochondrion to form a spherical crista.
