Fig. 1: Myc-TDP-25 is localized to mitochondria and damages mitochondria in mouse cortical neurons. | Cell Death & Disease

Fig. 1: Myc-TDP-25 is localized to mitochondria and damages mitochondria in mouse cortical neurons.

From: Nonmuscle myosin IIB regulates Parkin-mediated mitophagy associated with amyotrophic lateral sclerosis-linked TDP-43

Fig. 1

a Confocal images showing Myc-TDP-25 localization with GFP-Sec61, GFP-GalT, GFP-Tom20, or mitotracker in mouse cortical neurons. Scale bar, 10 μm. b The graphs show the fluorescence intensity profile across the arrow for green and red channels. c Schematic diagram of Myc-TDP-43, Myc-TDP-25, and deletion of mutant constructs. NES; Nucleus export sequence. d Confocal images showing the localization of either Myc-TDP-25, Myc-TDP-25 (ΔNES domain), or Myc-TDP-25 (ΔGly-rich domain) with mRFP-Tom20 in HeLa cells. Scale bar; 10 μm. e The graphs indicate the fluorescence intensity profile across the arrow for both green (Myc) and red (mitotracker) channels. f Electron microscopic cellular images in HEK293T cells expressing Myc vector or Myc-TDP-25. g The bar graph shows % of healthy or damaged mitochondria per cell. ***p < 0.0001. Values represent mean + SEM (n ≥ 11). h JC-1 analysis in mouse cortical neurons expressing CFP-TDP-25 without CCCP treatment. Control neurons expressing CFP were treated with either DMSO or 10 μM CCCP for 24 h. i The quantification graph shows the ratio of red/green fluorescence of JC-I. ***p < 0.001. Values represent mean + SEM (n ≥ 20).

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