Fig. 2: Myc-TDP-25 recruits Parkin and is removed by Parkin-mediated mitophagy. | Cell Death & Disease

Fig. 2: Myc-TDP-25 recruits Parkin and is removed by Parkin-mediated mitophagy.

From: Nonmuscle myosin IIB regulates Parkin-mediated mitophagy associated with amyotrophic lateral sclerosis-linked TDP-43

Fig. 2

a Confocal images showing cellular localization of Myc (control)/Myc-TDP-25, GFP-Parkin, or Tom20 in mouse cortical neurons. Scale bar; 10 μm. b The graphs show the fluorescence intensity profile across the arrow for green, red, and blue channels. c The bar graph represents the number of GFP-Parkin positive puncta per cell. ***p < 0.0001. Values represent mean + SEM (n ≥ 10). d Confocal images showing cellular localization of either LC3B, GABARAPL1, or p-p62 with Myc-TDP-25 in mouse cortical neurons. Scale bar; 10 μm. e The graphs represent the fluorescence intensity profile across the arrow for green (LC3B, GABARAPL1, and p-p62) and red channel (Myc-TDP-25). f Western blotting using HEK293T cell lysates expressing Myc-TDP-25 together with either control, Optineurin, NDP52, or TAX1BP siRNA with anti-Myc, anti-Optineurin, anti-NDP52, anti-TAX1BP, anti-GAPDH, or anti-H3 (histone 3) antibody. g The bar graph represents the knockdown efficiency of Optineurin, NDP52, or Tax1BP siRNAs or protein level of Myc-TDP-25 in the insoluble fraction in each group. *p < 0.05; **p < 0.001; ***p < 0.0001. Values represent mean + SEM (n ≥ 6).

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