Fig. 2: Gene expression profiling reveals that DHPS inhibition downregulates MYC.
From: Blockade of EIF5A hypusination limits colorectal cancer growth by inhibiting MYC elongation
a Volcano plot displaying the 148 differentially expressed genes in shDHPS vs. SCR. Axes show logarithmic transformation of fold change (x-axis) and p-values (FDR). Genes significantly upregulated (n = 79) and downregulated (n = 69) with an FDR < 0.05 and a log2(fold change) < −0.58 or >0.58 are represented in red and in dark blue, respectively. Labeled points indicate the first 25 upregulated and first 25 downregulated genes. b Barplot showing the log2(fold change, shDHPS vs. SCR) of the significantly upregulated (left panel) or downregulated (right panel) genes. Red bars indicate MYC target genes. c Western blotting of HCT116 infected with lentiviruses expressing shDHPS or SCR for 72 h, then selected with puromycin for 72 h. Staining for MYC, Hyp-EIF5A, EIF5A, DHPS, β-Catenin, pGSK3β, GSK3β, pERK, ERK, and p53. Vinculin, loading control. d Luciferase assay (n = 3) in HCT116 cells transfected for 24 h with TCF/LEF responsive reporter TOP or its negative control FOP. The day after transfection, cells were serum starved for 8 h and treated with 50 mM lithium chloride and 100 µM GC7 for other 24 h. For Statistical analysis: ns not significant, *p < 0.05, **p < 0.01, ***p < 0.001, by Student’s t-test. Data represent the mean ± SD of experiments performed in triplicates and repeated at least three times.
