Fig. 6: Expression of APAF1 is decreased upon EMC6 inhibition in PRSS1Tg AP and CP model.
From: EMC6 regulates acinar apoptosis via APAF1 in acute and chronic pancreatitis
Immunofluorescence staining to examine the co-localization of EMC6 (red fluorescence) and APAF1 (green fluorescence) in pancreatic tissues from PRSS1Tg mice AP model with EMC6 inhibition (A) or APAF1 (B) inhibition. Immunofluorescence staining to examine the co-localization of EMC6 (red fluorescence) and APAF1 (green fluorescence) in pancreatic tissues from PRSS1Tg mice CP model with EMC6 inhibition (C) or APAF1 inhibition (D). E Six AP samples from PRSS1Tg mice were immunoprecipitated with anti-EMC6 antibody and immunoblotted with the indicated antibodies, which was performed to examine whether EMC6 interacted with APAF1 in pancreatitis tissues. F qRT-PCR for analysis of the expression levels of ER stress markers ATF6, CHOP, calnexin, PDI, and IRE1α in pancreatic tissues from PRSS1Tg mice. Scale bars = 50 μm.
