Fig. 5: UBE2F in platinum-treated xenografts is significantly and inversely correlated with tumor weight and NOXA, respectively.
A Platinum treatment increases protein levels of UBE2F and NOXA in xenograft tumors. A549 cells (2 × 106) were injected subcutaneously into the flanks of nude mice. When the size of the tumor reaches about 20 mm2, mice were intraperitoneally injected with saline, cisplatin (4 mg/kg, once every 4 days, three cycles), or carboplatin (25 mg/kg, once every 2 days, six cycles). Protein levels of UBE2F, NOXA, RBX1, and RBX2 were determined by western blot and normalized against β-actin. B Platinum treatment has no effect on the UBE2F mRNA levels in xenograft tumors. Nude mice with xenografts were treated as in A. mRNA levels of UBE2F were determined by RT-PCR and normalized against β-actin. C, D Protein levels of UBE2F and NOXA are positively and negatively correlated with the weight of the xenograft tumors subjected to platinum, respectively. Nude mice with xenografts were treated as in A. At the end of the experiments, tumor xenografts were measured for weight. E UBE2F is inversely correlated with NOXA in xenograft tumors subjected to platinum.
