Fig. 4: Deacetylation at K62 promotes nuclear translocation of PKM2. | Cell Death & Disease

Fig. 4: Deacetylation at K62 promotes nuclear translocation of PKM2.

From: HDAC8-dependent deacetylation of PKM2 directs nuclear localization and glycolysis to promote proliferation in hepatocellular carcinoma

Fig. 4

A–D HepG2 cells were transfected with siHDAC8 (or negative control siRNA) or HA-HDAC8 plasmids (or vector plasmid), after 24 h, cells were placed on glass coverslips, cultured for another 24 h. Anti-PKM2 (red) and anti-k62ac (green) were used for immunofluorescence (scale bar: 50 μm) (A, C). The line profiles of the mean fluorescence intensity of PKM2 and DAPI signals were measured by ImageJ software. The remaining cell pellets were separated by cytosolic and nuclear fractions (B, D) for western blotting. E The cell lines which were stably expressing Flag-mcherry-PKM2 plasmids (WT/K62R/K62Q) in shPKM2-HepG2 cell lines. Immunofluorescence technique was used like above description (F), the remaining cell pellets were separated by cytosolic and nuclear fractions (G) for western blotting (***p < 0.001, ****p < 0.0001, one way ANOVA).

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