Fig. 3: Overexpression of cCNTNAP3 inhibits the growth of ESCC cells in vivo. | Cell Death & Disease

Fig. 3: Overexpression of cCNTNAP3 inhibits the growth of ESCC cells in vivo.

From: CircCNTNAP3-TP53-positive feedback loop suppresses malignant progression of esophageal squamous cell carcinoma

Fig. 3

a–c Eca-109 cells transfected with empty vector (pcDNA3.1) or cCNTNAP3 plasmids were subcutaneously injected into the armpit of nude mice (1 × 107 cells per mice, n = 5 each group). The volume and weight of subcutaneous xenograft tumors of ESCC cells isolated from nude mice. d The expression level of cCNTNAP3 in xenografted tumors was analyzed by qRT-PCR. e IHC staining showed the protein expression of Ki-67, CD-31, and p53 in xenograft tumor tissues. All the results were shown as mean ± SD (n = 3), which were three separate experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test).

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