Fig. 1: Circular RNA transcript of the XPO1 gene.
From: A novel circular RNA, circXPO1, promotes lung adenocarcinoma progression by interacting with IGF2BP1
(a) Localization of circXPO1 and back-splicing of exon 3 and exon 4. (b) Agarose gel electrophoresis analysis of PCR products of divergent primer and convergent primer. ←→ divergent primers; →← convergent primers. GAPDH was used as a control for a linear RNA transcript. cDNA: complementary DNA, gDNA: genomic DNA. (c) Relative expression level of XPO1 and circXPO1 in A549 and SPC-A1 after RNase R digestion. DMSO treated cells were used as a control and set to 1. *P < 0.05, **P < 0.01, P values were calculated with Mann–Whitney U test. (d) Expression of circXPO1 in normal lung cells (HBE) and lung cancer cell lines. HBE was used as a reference and set to 1. (e) Dot plot of XPO1 gene copy number and circXPO1 gene expression. P value was calculated by Spearman correlation.
