Fig. 4: CD4 T cells treated with KML001 exhibit telomere erosion, disruption of the BER pathway, and p53 activation. | Cell Death & Disease

Fig. 4: CD4 T cells treated with KML001 exhibit telomere erosion, disruption of the BER pathway, and p53 activation.

From: Telomeric injury by KML001 in human T cells induces mitochondrial dysfunction through the p53-PGC-1α pathway

Fig. 4

A Telomere length in CD4 T cells exposed to KML001 or control for 72 h was measured by flow-FISH (n = 5). B Representative images and summary data of the percentage (%) of chromosomes with telomere free ends in CD4 T cells treated with KML001 or DPBS in the presence of anti-CD3/CD28 antibody stimulation for 72 h, and then analyzed by meta-FISH. Approximately 50 cells in metaphase were counted using confocal microscopy (scale bar is 5 μm). C Summary data for qPCR of telomeric DNA content following exposure of CD4 T cells to KML001 for 5 days and DNA glycosylase Fpg (n = 17). D Representative image of the Southern blot of telomeric DNA following exposure of CD4 T cells to KML001 for 5 days and DNA glycosylase Fpg. E Representative images and summary data of western blot analysis of the BER-related XRCC1 and NEIL1 expressions in CD4 T cells treated with KML001 or DPBS control for 6, 12, 24, and 48 h (XRCC1, n = 4; NEIL1, n = 3). F Confocal microscopy representative images and summary data (n = 4) of the co-localization of OGG1 with TRF1 in CD4 T cells treated with KML001 or DPBS control for 48 h (scale bar is 1 μm). All summary data are shown as mean ± SEM.

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