Fig. 4: Knockdown of NCAPH expression dramatically impaired the tumor growth, invasion and EMT process in nude mice. | Cell Death & Disease

Fig. 4: Knockdown of NCAPH expression dramatically impaired the tumor growth, invasion and EMT process in nude mice.

From: HPV E7-mediated NCAPH ectopic expression regulates the carcinogenesis of cervical carcinoma via PI3K/AKT/SGK pathway

Fig. 4

A Fluorescence microscopy of successful infection of HeLa cells with lentivirus containing pGV248-NCAPH shRNA (siNCAPH) and pGV248 control vector (LV-NC). Scale bars, 100 μm. B Western blot analysis confirmed the knockdown of NCAPH in HeLa cells after 6-weeks of selection using puromycin. C, D The selected HeLa cells were injected into the axillary fossa of the mice. Ten weeks after injection, GFP expressing tumors were imaged in live mice. Then mice were humanely killed, and tumor volumes were calculated (six mice in each group). Representative images are shown. E The expression of NCAPH in tumors was determined by real-time PCR. F HE staining showed that tumors in the LV-NC group exhibited obvious muscle invasion, but those in the siNCAPH group were well-encapsulated. Scale bars, 100 μm. G Immunohistochemical staining showed that ZO-1 protein signal was enhanced while vimentin was weakened after knockdown of NCAPH expression in mice tumors. Scale bars, 100 μm. Data are presented as mean ± SD (n = 3). * represents p < 0.05, ** represents p < 0.01.

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