Fig. 2: Downregulation of Linc00426 leads to significantly inhibit proliferation, metastasis, and invasion of LUAD in vitro.
From: Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge
a Quantification of Linc00426 in A549 cells after infection with sh-NC and three different shRNA (sh-Linc00426-1#, sh-Linc00426-2#, and sh-Linc00426-3#). RT-qPCR was employed to examine the infection efficiency. b Cell proliferation was detected by Celigo Image Cytometer on the reading board once a day for 5 days. Scale bar = 500 μm. c Effects of Linc00426 knockdown on cellular mobility detected by wound-healing assay. Photos were taken by Celigo Image Cytometer at 0, 24, and 32 h, respectively. Scale bar = 500 μm. d Effects of Linc00426 knockdown on invasion ability determined using Transwell assay. Photos were taken by optical microscope at 24 and 32 h, respectively. Scale bar = 100 μm. e Expression levels of EMT-related markers were detected by Western blot in A549 cells infected with sh-NC and sh-Linc00426. f Expression of EMT-related markers in A549 cells with sh-NC and sh-Linc00426 by immunofluorescence analysis. The nuclei were counterstained using DAPI. Scale bar = 50 μm. Experiments were performed three times and data were presented as mean ± SD. *P < 0.05. Student’s t test.
