Fig. 3: Linc00426 is required for cytoskeleton rearrangement and matrix metalloproteinase expression and downregulation of Linc00426 represses tumor growth and metastasis of LUAD in vivo.
From: Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge
a The expression of MMP-2 and MMP-9 in A549 cells with sh-NC and sh-Linc00426. b Images of F-actin (red) and nucleus (blue) staining in A549 cells with sh-NC and sh-Linc00426. The chart shows F-actin polymerization in A549 cells with sh-NC and sh-Linc00426. Scale bar = 50 μm. c The expression of p-LIMK and p-cofilin in A549 cells with sh-NC and sh-Linc00426. d Growth curves of A549 cells with sh-NC and sh-Linc00426 derive from subcutaneous tumor xenografts. Tumor volume is measured with time duration. e Tumor weight is measured after surgical dissection. f Number of metastatic nodules in different tissue slices. g Bright-field imaging (arrows indicate metastases) and counting of metastatic nodules. h H&E staining and number of metastatic foci in the lungs injected with sh-NC and sh-Linc00426 cells. The metastatic focus is indicated with dotted line and black arrows; The alveolar is indicated with blue arrows. Scale bar = 40 μm and 10 μm. *P < 0.05. Student’s t test.
