Fig. 7: Validation of kcnq1ot1 as a ceRNA for miR-452-3p. | Cell Death & Disease

Fig. 7: Validation of kcnq1ot1 as a ceRNA for miR-452-3p.

From: LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis

Fig. 7

A Schematic representation of miR-452-3p binding site in the kcnq1ot1 sequence and corresponding mutation. B The luciferase reporter plasmids kcnq1ot1-WT or kcnq1ot1-Mut were co-transfected into 293T cells with miR-452-3p mimic or its negative control for 48 h, followed by detection of luciferase activity. C THP-1 macrophages were transfected with bio-NC, bio-miR-452-3p-WT or bio-miR-452-3p-Mut for 48 h, which was followed by pull-down assay. D THP-1 macrophages were incubated with miR-452-3p mimic/inhibitor or their negative controls for 48 h. The expression of kcnq1ot1 was evaluated by qRT-PCR. E Following transduction of THP-1 macrophages with PBS, LV-NC, or LV-kcnq1ot1 for 72 h, qRT-PCR was conducted to detect miR-452-3p expression. Data are represented as mean ± SD from three independent experiments. **P < 0.01, ***P < 0.001; ns not significant.

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