Fig. 8: Kcnq1ot1 sponges miR-452-3p to increase HDAC3 levels and inhibit ABCA1 expression. | Cell Death & Disease

Fig. 8: Kcnq1ot1 sponges miR-452-3p to increase HDAC3 levels and inhibit ABCA1 expression.

From: LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis

Fig. 8

A, B qRT-PCR analysis of miR-452-3p expression in the aorta (n = 10) and MPMs (n = 5) from apoE−/− mice. C–E THP-1 macrophages were transfected with miR-452-3p mimic for 48 h and then transduced with LV-kcnq1ot1 for 72 h (n = 3). C, D The mRNA and protein levels of HDAC3 and ABCA1 were detected by qRT-PCR and western blot, respectively. E Detection of cholesterol efflux using a liquid scintillation counter. F Schematic overview of the proatherosclerotic action of kcnq1ot1. Kcnq1ot1 increases the levels of HDAC3 by functioning as a ceRNA for miR-452-3p, which in turn downregulates ABCA1 expression, inhibits cholesterol efflux from macrophage, reduces plasma HDL-C levels, and exacerbates atherosclerosis. Data were represented as mean ± SD. **P < 0.01, ***P < 0.001 vs. control group; ##P < 0.01, ###P < 0.001 vs. LV-kcnq1ot1 group.

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