Fig. 4: ATF3 is required for the activation of primary HSCs.
From: Transcriptional factor ATF3 promotes liver fibrosis via activating hepatic stellate cells
a Primary HSCs were infected with ATF3-shRNA1/2 for 72 h. The expression of α-SMA, Col1α1, Col3α1, Col4α5, Mmp2, Mmp9, and Timp1 was detected by qRT-PCR; *p < 0.05. b The protein level of α-SMA, COL1, and MMP2 were detected by western blot. GAPDH was used as an internal control. c The mRNA level of α-SMA, Col1α1, Col3α1, Col4α5, Mmp2, Mmp9, and Timp1 were detected in primary HSCs infected with lenti-ATF3 or lenti-control by qRT-PCR; *p < 0.05. d The protein level of α-SMA, COL1, and MMP2 were detected in ATF3 upregulated primary HSCs by western blot. GAPDH was used as an internal control. The data were expressed as the mean ± SEM for at least triplicate experiments.
