Fig. 1: SOX9 is upregulated in CRC cells and promotes cell growth, stemness, migration, and invasion. | Cell Death & Disease

Fig. 1: SOX9 is upregulated in CRC cells and promotes cell growth, stemness, migration, and invasion.

From: SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9

Fig. 1

A GEPIA data showed the expression of SOX9 in 275 COAD (colon adenocarcinoma) samples and 349 normal colon tissues. B The qRT-PCR and western blot experiments were utilized to detect the expression of SOX9 in CRC cell lines and normal NCM-460 cells. C The knockdown efficiency of SOX9 in SW480 and SW1116 cells was tested by qRT-PCR and western blot experiments. D, E The proliferation capability of cells was detected by colony formation assay and EdU assay (scale bar = 150 μm). F Flow cytometry was utilized to test the apoptotic cells marked with FITC-Annexin-V or together with FITC-Annexin-V and PI. G Tumor sphere-formation assay was utilized to detect cell stemness (scale bar = 75 μm). H, I Transwell assay was adopted to test cell migration and invasion under different contexts (scale bar = 250 μm). *P < 0.05, **P < 0.01.

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