Fig. 2: SOX9 binds to FARSA-AS1 promoter. | Cell Death & Disease

Fig. 2: SOX9 binds to FARSA-AS1 promoter.

From: SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9

Fig. 2

A The expression of top 20 genes correlated with SOX9 in COAD samples from GEPIA database was detected by qRT-PCR. B The qRT-PCR was adopted to test the effects of silencing SOX9 on the expression of NOS2, FOS, PVRL1, and FARSA-AS1. C FARSA-AS1 expression in CRC cells was tested by qRT-PCR. D The effect of overexpressing SOX9 on the expression of FARSA-AS1 in SW480 and SW1116 cells was detected by qRT-PCR. E The DNA motif for SOX9 was obtained from JASPAR. F SOX9 binding sites in FARSA-AS1 promoter were predicted by JASPAR. G Luciferase reporter assay was utilized to detect the combined situation of SOX9 in FARSA-AS1 promoter. H, I ChIP and DNA pull-down assays were adopted to prove the combination between SOX9 and FARSA-AS1 promoter. *P < 0.05, **P < 0.01.

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