Fig. 4: FARSA-AS1 upregulates SOX9 by sponging miR-18b-5p. | Cell Death & Disease

Fig. 4: FARSA-AS1 upregulates SOX9 by sponging miR-18b-5p.

From: SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9

Fig. 4

A The effect of silencing FARSA-AS1 on SOX9 expression was tested by qRT-PCR and western blot experiments. B, C FISH assay (scale bar = 10 μm) and subcellular fractionation assay were conducted to test the location of FARSA-AS1 in SW480 and SW1116 cells. D Two miRNAs shared by FARSA-AS1 and SOX9 were picked out through DIANA and starBase. E The expressions of miR-18a-5p and miR-18b-5p were detected in CRC cells by qRT-PCR. F The binding sites between miR-18b-5p and FARSA-AS1/SOX9. G, H Luciferase reporter and RNA pull-down experiments were adopted to prove the binding between miR-18b-5p and FARSA-AS1. I, J The interaction between miR-18b-5p and SOX9 was detected through luciferase reporter and RNA pull-down experiments. **P < 0.01.

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