Fig. 6: FARSA-AS1 upregulates FARSA via sequestering miR-28-5p. | Cell Death & Disease

Fig. 6: FARSA-AS1 upregulates FARSA via sequestering miR-28-5p.

From: SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9

Fig. 6

A The expression of FARSA was tested by qRT-PCR and western blot analyses in CRC cells after knocking down FARSA-AS1. B, C The qRT-PCR and western blot were adopted to detect the expression of FARSA in CRC cells. D Two shared miRNAs of FARSA-AS1 and FARSA were found by DIANA and starBase. E The expression of miR-28-5p and miR-708-5p in CRC cells was detected by qRT-PCR. F The binding sequence between miR-28-5p and FARSA-AS1 and that between FARSA and miR-28-5p were shown. G The overexpression efficiency of miR-28-5p was tested by qRT-PCR. H, I The experiments of luciferase reporter and RNA pull-down were conducted to confirm the binding between miR-28-5p and FARSA-AS1. J, K The combination of miR-28-5p with FARSA was confirmed through luciferase reporter experiment and RNA pull-down assay. *P < 0.05, **P < 0.01.

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