Fig. 7: FARSA-AS1 enhances CRC progression by upregulating SOX9 and FARSA. | Cell Death & Disease

Fig. 7: FARSA-AS1 enhances CRC progression by upregulating SOX9 and FARSA.

From: SOX9-activated FARSA-AS1 predetermines cell growth, stemness, and metastasis in colorectal cancer through upregulating FARSA and SOX9

Fig. 7

A The qRT-PCR was conducted to test the overexpression efficiency of FARSA in SW480 and SW1116 cells. B, C The capability of cell proliferation was tested by EdU experiment and colony formation experiment. D Flow cytometry experiment was carried out to test cell apoptosis. E Tumor sphere-formation assay was carried out for detecting cell stemness. F, G The capability of cell migration and invasion was estimated via Transwell assay. H The representative pictures and the growth curves of tumors derived from sh-NC- or sh-FARSA-AS1-transfected CRC cells. I Tumor weight was estimated in above two groups. J The expressions of FARSA-AS1, SOX9, and FARSA were detected separately by qRT-PCR in tumors with or without silenced FARSA-AS1. K The representative images of HE staining (scale bar = 150 μm) of lung from indicated groups, and the quantification bar graph of lung metastatic nodules in tumors with or without silenced FARSA-AS1 were shown. *P < 0.05, **P < 0.01.

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