Fig. 3: Downregulation of TRAF3 reduced neuronal apoptosis and ameliorated neurological deficits. | Cell Death & Disease

Fig. 3: Downregulation of TRAF3 reduced neuronal apoptosis and ameliorated neurological deficits.

From: TRAF3 mediates neuronal apoptosis in early brain injury following subarachnoid hemorrhage via targeting TAK1-dependent MAPKs and NF-κB pathways

Fig. 3

A Representative western blot images of Bcl-xL, Bax, Caspase-3, and Cleaved caspase-3. Quantitative analysis showed that downregulation of TRAF3 increased the expression of Bcl-xL (B) and Caspase-3 (D), while decreased the expression of Bax (C) and Cleaved caspase-3 (E) compared with SAH and SAH + control siRNA groups. N = 6 per mice group. *p < 0.05 versus the sham group, #p < 0.05 versus the SAH group. F Double staining of TUNEL (red) and NeuN (green); nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. G The TUNEL-positive index of neuron in the temporal lobe was increased in the SAH and SAH + control siRNA groups, TRAF3 siRNA treatment significantly decreased the number of TUNEL-positive cells in SAH mice at 24 h post-SAH. N = 6 mice per group. *p < 0.05 versus the sham group, #p < 0.05 versus the SAH group. H, I Nissl staining results showed that TRAF3 siRNA treatment significantly decreased the percentage of necrotic neurons at 7 days post-SAH. Scale bar: 20 μm. N = 6 mice per group. *p < 0.05 versus the sham group, #p < 0.05 versus the SAH group. J Downregulation of TRAF3 could ameliorate motor coordination scores on days 3 and 7 post-SAH. K The RR time on days 3 and 7 post-SAH in the SAH and SAH + control siRNA groups were lower compared to the sham group. The RR time was increased by TRAF3 siRNA treatment. N = 10 mice per group. Data represent mean ± SEM. *p < 0.05 versus the sham group, #p < 0.05 versus the SAH group.

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