Fig. 5: Blocking the Akt-mTOR and metabolic pathways in HBV/TLR2-L stimulates B cells.
From: Hepatitis B virus particles activate B cells through the TLR2–MyD88–mTOR axis
A Purified B cells were stimulated with TLR2-L/HBV particles for 24 h with or without metabolic pathway inhibitors. B, C MHC II and CD86 expression in B cells was detected by flow cytometry following treatment with Rapamycin/Akti-1/2. Glucose and glutamine uptake was measured by detecting the MFI of the glucose analog 2-NBDG and glutamine transporter CD98 following treatment with Rapamycin/Akti-1/2. D, F MHC II and CD86 expression in B cells was detected by flow cytometry following metabolic blocking. E, G Akt, and p-mTOR expression were detected by flow cytometry following treatment with 2-DG/DON/oligomycin. Data are representative of three independent experiments. All data are presented as the mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001) according to one-way ANOVA.
