Fig. 6: HBV-enhanced B-cell function and metabolism are MyD88-dependent.
From: Hepatitis B virus particles activate B cells through the TLR2–MyD88–mTOR axis
Purified B cells were derived from WT, TLR2−/−, TRIF−/−, MyD88−/−, and TRIF/MyD88−/− mice and stimulated with HBV particles for 24 h. A TLR2 expression in B cells was detected by flow cytometry in WT, TLR2−/−, TRIF−/−, MyD88−/−, and TRIF/MyD88−/− B cells. B, C B-cell size and activation were assessed by FSC-A, MHC II, and CD86. D Glucose uptake was measured by detecting the MFI of the glucose analog 2-NBDG in WT, TLR2−/−, TRIF−/−, MyD88−/−, and TRIF/MyD88−/− B cells after 24 h of stimulation. E Akt and p-mTOR expression were detected by FACS in WT, TLR2−/−, TRIF−/−, MyD88−/−, and TRIF/MyD88−/− B cells after 24 h of stimulation (*p < 0.05; **p < 0.01; ***p < 0.001). Data are representative of three independent experiments. All data are presented as the mean ± SD (*p < 0.05; **p < 0.01; ***p < 0.001) according to two-way ANOVA.
