Fig. 3: In situ illustration of neuronal EVs derived miR-98 internalized into microglia after ischemic stroke. | Cell Death & Disease

Fig. 3: In situ illustration of neuronal EVs derived miR-98 internalized into microglia after ischemic stroke.

From: Neuronal extracellular vesicle derived miR-98 prevents salvageable neurons from microglial phagocytosis in acute ischemic stroke

Fig. 3

a The injection of control virus pAAV-SYN-MCS-EYFP-3FLAG (9.46 × 1012, 0.5 μL) into cortex of P20 C57BL/6 J mice (AP:-1mm, ML:-1.8 mm, DV:-0.2 mm). b The identification of the region and efficiency of virus infection (left, Scale bar = 200 μm) and the 3D reconstructions of Z-stack images in white dotted box acquired by a two-photon Microscope (right, 100 μm thickness). c pAAV-SYN-CD63-mCherry-3FLAG (1.81 × 1013, 0.3 μL) and pAAV-SYN-EYFP-3FLAG-miR-98 (8.36 × 1012, 0.3 μL) were simultaneously injected into the same coordinates of control virus and subsequent tMCAO model 3 weeks later. d The representative confocal images of CD63-mCherry co-labeled with miR-98-EYFP and DAPI on paraffin slices (5 μm thickness, Scale bar = 200 μm). e–g The representative confocal images, amplification in white dotted box and colocalization analysis, and 3D reconstructions of CD63-mCherry co-labeled with miR-98-EYFP and IBA1 on paraffin slices 6 h after ischemic stroke acquired by a confocal microscopy. (5 μm thickness, Scale bar = 200 μm) (n = 3 independent experiments). The white arrows represent the CD63-mCherry and miR-98-EYFP were co-labeled.

Back to article page