Fig. 2: Blocking BCL-XL can enhance cell inhibition by DHT. | Cell Death & Disease

Fig. 2: Blocking BCL-XL can enhance cell inhibition by DHT.

From: Suppressing BCL-XL increased the high dose androgens therapeutic effect to better induce the Enzalutamide-resistant prostate cancer autophagic cell death

Fig. 2

A Cell colony assay shows that cell colony number changs, but size does not change. B–C Cell colony assay shows EnzR cells were treated with DHT 50 nM for 2–10 days followed by changing to regular medium (2 nM DHT, 10 μM Enzalutamide) for the remainder of the time period. D BCL-2 and BCL-XL protein expression in EnzR-C4-2 and EnzR-C4-2B cell line with 50 nM DHT. E–F Cell colony assay shows cell growth with 50 nM DHT after transduction of oe-BCL-2 in EnzR-C4-2 (E) and EnzR-C4-2B (F) cell line. G–H Cell colony assay shows that cell growth with 50 nM DHT after transduction of sh-BCL-XL in EnzR-C4-2 (G) and EnzR-C4-2B (H) cell line. I–J Cell colony assay shows cell growth with 50 nM DHT with/without 10 μM WHEI-539 in EnzR-C4-2 (I) and EnzR-C4-2B (J) cell line. Data are presented as means ± SD. *p < 0.05 was considered statistically significant by students’ t-test for two groups or ANOVA for more than two groups.

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