Fig. 4: The effect of miRNA-9-5p inhibitor on BBB stability and synaptogenesis in vitro. | Cell Death & Disease

Fig. 4: The effect of miRNA-9-5p inhibitor on BBB stability and synaptogenesis in vitro.

From: Downregulation of microRNA-9-5p promotes synaptic remodeling in the chronic phase after traumatic brain injury

Fig. 4

A Schematic of the NVU. B The procedure used to reconstruct the NVU in vitro. C BMECs and D astrocytes visualized by scanning electron microscopy (bar = 30 μm). Quantitative data of the effects of the miRNA-9-5p inhibitor on E TEER, F γ-GT activity, and G EB and H HRP permeability in vitro (n = 5/group). I The immunofluorescence (bar = 50 μm) and J quantitative data of the tight junction structure in vitro. K The immunofluorescence (bar = 10 μm) and quantitative data of L PSD-95 and M synaptotagmin in neurons. The numbers of N synaptic puncta and O synapses of neurons (n = 10/group) (*P < 0.05, ***P < 0.001 versus the control group; #P < 0.05, ##P < 0.01 versus the ACM group; ++P < 0.01, +++P < 0.001 versus the ACM (inhibitor) group) (ACM: astrocyte co-cultured model, inh: inhibitor, siR: siRNA). Error bars indicate mean ± SD.

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