Fig. 2: The expression levels of hsa_circ_0007456 in HCC were associated with their susceptibility to NK cells. | Cell Death & Disease

Fig. 2: The expression levels of hsa_circ_0007456 in HCC were associated with their susceptibility to NK cells.

From: Hsa_circ_0007456 regulates the natural killer cell-mediated cytotoxicity toward hepatocellular carcinoma via the miR-6852-3p/ICAM-1 axis

Fig. 2

After infection with hsa_circ_0007456-overexpressing or shRNA lentivirus or the control lentivirus in SMMC-7721 or QGY-7703: A–D The expression levels of hsa_circ_0007456 in the infected cells were determined by RT-qPCR; E–H The NK cell cytotoxicity to the infected cells was assessed by calcein release assays in indicated E/T ratios; I–L The numbers of perforin-containing NK cells against the infected cells were counted and analyzed; M–P The conjugate formation between NK cells with the infected cells was assessed by conjugation assay. Data are representative of three independent experiments and are presented as mean ± SD. *p < 0.05; **p < 0.01.

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