Fig. 3: miR-6852-3p is the direct target of hsa_circ_0007456. | Cell Death & Disease

Fig. 3: miR-6852-3p is the direct target of hsa_circ_0007456.

From: Hsa_circ_0007456 regulates the natural killer cell-mediated cytotoxicity toward hepatocellular carcinoma via the miR-6852-3p/ICAM-1 axis

Fig. 3

A, B The relative expression levels of hsa_circ_0007456 in nuclear and cytoplasm of SMMCC-7721 (A) or QGY-7703 (B) were quantified by RT-qPCR. C The relative levels of hsa_circ_0007456 were quantified by RT-qPCR after pull-down with indicated microRNA probes. D–G After pull-down with hsa_circ_0007456 specific probe or the control probe in SMMCC-7721 or QGY-7703 cell lysis, the levels of hsa_circ_0007456 (D, E) and miR-6852-3p (F, G) were respectively quantified by RT-qPCR. H, I After pull-down biotin-coupled miR-6852-3p or the control probe in SMMCC-7721 or QGY-7703 cell lysis, the enrichment levels of the hsa_circ_0007456 were quantified by RT-qPCR. J The predicted binding motif between hsa_circ_0007456 and miR-6852-3p is indicated in red, and the mutant hsa_circ_0007456 was designed without the complementary sequence. K, L SMMCC-7721 or QGY-7703 stably expressing the luciferase construct containing the wild-type (WT) or mutant (MT) hsa_circ_0007456 were respectively transfected with miR-6852-3p mimic, inhibitor or the negative control (NC), and then the luciferase activity was examined. M, N The regression analysis of correlation between the expression of hsa_circ_0007456 and miR-6852-3p in HCC tissues (M) and portal vein tumor thrombus (N). Data are representative of three independent experiments and are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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