Fig. 7: The hsa_circ_0007456 regulated the susceptibility of HCC to NK cells by targeting miR-6852-3p/ICAM-1 axis.

A–D After treatment with the indicated hsa_circ_0007456-overexpressing or the control lentivirus plus miR-6852-3p mimic or negative control (NC) in SMMCC-7721 or QGY-7703, the NK cell cytotoxicity to the transfected cells was assessed by calcein release assays in indicated E/T ratios (A, B), while the numbers of perforin-containing NK cells against to the transfected cells were counted and analyzed (C, D). E–H After treatment with the indicated hsa_circ_0007456-shRNA or the control lentivirus plus miR-6852-3p inhibitor or negative control (NC) in SMMCC-7721 or QGY-7703, the NK cell cytotoxicity to the transfected cells was assessed by calcein release assays in indicated E/T ratios (E, F), while the numbers of perforin-containing NK cells against to the transfected cells were counted and analyzed (G, H). I–L After treatment with the indicated hsa_circ_0007456-overexpressing or the control lentivirus plus ICAM-1 siRNA or negative control (NC) in SMMCC-7721 or QGY-7703, the NK cell cytotoxicity to the transfected cells was assessed by calcein release assays in indicated E/T ratios (I, J), while the numbers of perforin-containing NK cells against to the transfected cells were counted and analyzed (K, L). M–P After treatment with the indicated hsa_circ_0007456-shRNA or the control lentivirus plus pcDNA3.1-ICAM-1 or negative control (NC) in SMMCC-7721 or QGY-7703, the NK cell cytotoxicity to the transfected cells was assessed by calcein release assays in indicated E/T ratios (M, N), while the numbers of perforin-containing NK cells against to the transfected cells were counted and analyzed (O, P). Data are representative of three independent experiments and are presented as mean ± SD. **p < 0.01.