Fig. 2: Cellular function and in vivo experiments. | Cell Death & Disease

Fig. 2: Cellular function and in vivo experiments.

From: KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer

Fig. 2

A The proliferation abilities of PC3 and C42B cells transfected with KDM6B siRNA are shown. Error bars means SD, N = 3 independent repeats. B The colony formation abilities of PC3 and C42B cells transfected with KDM6B siRNA are shown. C, D The knockdown of KDM6B by siRNA suppressed the migration and invasion abilities of PC3 and C42B cells. Error bars means SD, n = 3 independent repeats. E A cell cycle analysis of PC3 and C42B cells transfected with KDM6B siRNA was performed. F An apoptosis analysis of PC3 and C42B cells transfected with KDM6B siRNA was performed. G Nude mice were subcutaneously xenografted with PC3 and C42B cells, and were treated intratumorally with scrambled or KDM6B siRNA. The tumor growth curve and representative tumor images are shown (Note: AR, androgen receptor; TF, transcription factor; DHT, dihydrotestosterone; MDV3100, enzalutamide; DMSO, dimethylsulfoxide; WT, wild type; MUT, mutation.) Error bars mean SD, six mice in each group with one lost in PC3 cell line + negative control siRNA (*p < 0.05, ***p < 0.001).

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