Fig. 5: KDM6B expression is downregulated by ARs. | Cell Death & Disease

Fig. 5: KDM6B expression is downregulated by ARs.

From: KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer

Fig. 5

A A correlation analysis between KDM6B and ARs using data from GEO:GSE46602 (n = 50, p = 0.83, r = -0.03), GEO:GSE62872 (n = 424, p = 0.18, r = −0.07), Dingtianlidi sequencing program (n = 271, p = 0.02, r = −0.18) and the tissue microarray (n = 156, p = 0.33, r = −0.08) was performed. B Representative images of the KDM6B and AR immunohistochemistry analysis indicate a negative correlation between KDM6B and ARs. C Comparisons of the KDM6B mRNA and protein expression levels among the DMSO, DHT, and MDV3100 treatments in the LNCaP cell line were performed by PCR and western blot analyses. Error bars means SD, n = 3 independent repeats. D Comparisons of the AR-binding levels to the KDM6B promoter among the DMSO, DHT, and MDV3100 treatments in the LNCaP cell line were performed using a ChIP-PCR analysis. Error bars means SD, n = 3 independent repeats. E Dual-luciferase reporter assay was used to verify that AR directly regulated KDM6B transcription by 3′-UTR. Error bars means SD, n = 3 independent repeats (Note: AR, androgen receptor; DHT, dihydrotestosterone; MDV3100, enzalutamide; DMSO, dimethylsulfoxide) (ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001).

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