Fig. 6: KDM6B demethylates H3K27me3 on the promoter of CCND1 and cooperates with smad2/3 to prompt the expression of CCND1. | Cell Death & Disease

Fig. 6: KDM6B demethylates H3K27me3 on the promoter of CCND1 and cooperates with smad2/3 to prompt the expression of CCND1.

From: KDM6B is an androgen regulated gene and plays oncogenic roles by demethylating H3K27me3 at cyclin D1 promoter in prostate cancer

Fig. 6

A A supervised hierarchical clustering of the genes differentially expressed between the C42B wild-type samples and the KDM6B depletion C42B samples is shown. B A correlation analysis between KDM6B and CCND1 using data from GEO:GSE46602 (n = 50, p = 0.05, r = 0.28), GEO:GSE62872 (n = 424, p = 0.001, r = 0.15), Dingtianlidi sequencing program (n = 271, p < 0.001, r = 0.34) and the tissue microarray (n = 176, p = 0.002, r = 0.23) was performed. C Representative images of the KDM6B and CCND1 immunohistochemistry results indicate a positive correlation between KDM6B and CCND1. D Comparisons of the CCND1 mRNA and protein expression levels among the siRNA-NC, siRNA-KDM6B, DMSO, and GSK-J4 treatments in the PC3 cell line were performed by PCR and western blot analyses. Error bars mean SD, n = 3 independent repeats. E A ChIP-PCR analysis demonstrates that the depletion and inhibition of KDM6B increases H3K27me3 on the CCND1 promoter in the PC3 cell line. Error bars mean SD, n = 3 independent repeats. F Immunoprecipitation-based mass spectrometry revealed the interaction between KDM6B and Smad2/3. G An immunoprecipitation analysis indicates that treatments with siRNA-KDM6B and GSK-J4 reduce the amount of interaction between KDM6B and Smad2/3. H Comparisons of the KDM6B and Smad2/3-binding levels to the CCND1 promoter among the siRNA-NC, siRNA-KDM6B, DMSO, and GSK-J4 treatments in the PC3 cell line were performed using a ChIP-PCR analysis. Error bars mean SD, n = 3 independent repeats (Note: DMSO, dimethylsulfoxide, ns: not significant, **p < 0.01, ***p < 0.001).

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