Fig. 4: DDX39B upregulates and correlates to FUT3 expression.
From: The DDX39B/FUT3/TGFβR-I axis promotes tumor metastasis and EMT in colorectal cancer
A, B Enrichment analysis using GSEA and GO showed RNA export and splice in CRC were correlated with DDX39B expression. C Relationship of certain RNA processes with low/high DDX39B expression was shown using GSE17538 (n = 177). D RNA-seq results showed upregulated and downregulated transcripts with DDX39B overexpression. E qRT-PCR was conducted to validate the fold changes in several gene expressions (tumor-related) in SW480/Vector and SW480/DDX39B groups. F Fold changes of FUT expression with DDX39B overexpression (RNA-seq data). G–I Expression levels of FUT3 mRNA and protein in CRC cells with DDX39B overexpression or knockdown; error bars, SD. J, K Correlation between DDX39B and FUT3 mRNA expression in 12 CRC/normal colon cells and in 52 paired CRC tissues. L IHC and IF assay using serial sections of paired CRC tissues were performed to analyze the correlation between FUT3 and DDX39B expression; scale bars, 50 μm.
