Fig. 3: Legumain deficiency inhibits IRI-induced tubular ferroptosis.
From: Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI
Collection of kidney samples was performed as described in Fig. 1 (n = 8). A & B Quantification of kidney Acsl4 and Cox-2 mRNA levels by qPCR. C Level of intracellular ROS in kidneys measured by the fluorescent probe carboxy-H2DCFDA. D Representative images of the fluorescence assay (left panel) and quantification (right panel) of ROS in kidneys using the fluorescent probe DHE (red), DAPI (blue) was used to stain the nuclei. Scale bar, 50 μm. E Levels of lipid peroxidation in kidneys were measured by MDA assay. F Quantification of kidney GPX4 mRNA levels by qPCR. G Protein levels of GPX4 in kidney samples assessed by western blotting. Statistical results are shown in the right panel. All data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; two-way ANOVA.
