Fig. 1: The effects of TRIM21 on OS cell autophagy. | Cell Death & Disease

Fig. 1: The effects of TRIM21 on OS cell autophagy.

From: TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy

Fig. 1

A OS cells transfected with HA-TRIM21 or HA-vector were collected for western blotting assay. B U2-OS cells stably expressing H125-TRIM21 or control H125-V were treated with TET (10 μg/ml, 12 h) or in combinations with CQ (5 μM, 2 h) as indicated. C OS cells transfected with si-TRIM21 or si-NC (control) were collected for western blotting assay. D U2-OS cells were transfected with si-TRIM21 along with its control and treated with CQ (5 μM, 2 h) as indicated. Then, the cells of A–D were collected for western blotting assay with the indicated antibodies. The corresponding quantitative analyses of LC3-II/GAPDH or p62/GAPDH were shown in their bottom panels, respectively (n = 3, SEM). E U2-OS cells stably expressing H125-TRIM21 or control H125-V were transfected with the mCherry-EGFP-LC3 plasmid and treated with TET (10 μg/ml, 12 h) to induce the expression of TRIM21. U2-OS cells stably knocking down TRIM21 (sh-TRIM21#1) or control sh-NC were transfected with the mCherry-EGFP-LC3 plasmid. Then the cells were subjected to IF assay. DAPI staining was used to mark the nucleus. Scale bar: 10 μm. Arrows: the red or yellow dots (upper panel). Lower panel: quantitative analysis of red-only dots/yellow dots of the merged images in 60 cells. F U2-OS cells stably expressing H125-TRIM21 or control H125-V were treated with TET (10 μg/ml, 12 h) or in combinations with CQ as indicated. Then the cells were performed western blotting assay. Quantitative analysis of the p62/GAPDH ratio was shown in the bottom panel (n = 3, SEM). *p < 0.05, **p < 0.01. All data were representative of three independent experiments.

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